Aung San Suu Kyi and her National League for Democracy (NLD) were removed by the military in a February coup that sparked a mass uprising and a brutal crackdown on dissent.
She pleaded not guilty to charges of incitement, her lawyer Khin Maung Zaw said on Tuesday, adding she “seemed to be in good health”, a week after she skipped a separate hearing because she felt unwell.
Deposed President Win Myint has also pleaded not guilty to incitement.
Tuesday saw the court hear prosecution testimony that Aung San Suu Kyi flouted coronavirus restrictions during elections her party won by a landslide last year, her lawyer said.
Next month she will face a new trial on corruption charges, and she has also been charged with violating a colonial-era secrecy law, although this has also yet to come to court.
Her NLD government was deposed by the military over unsubstantiated allegations of voter fraud during the 2020 polls, in which it trounced a political party aligned with the generals.
Security forces have killed more than 1,100 civilians since the coup, according to a local monitoring group.
The military says the death toll is much lower.